Toyobo ligation high ver 2
Web2-3. ligation of annealed oligo and gRNA expression plasmid Ligation high (1/2 volume of DNA) 1 ul Bbs ... 1/100 diluted annealed oligo 1 ul / 3 ul e.g. (TOYOBO Ligation high ver.2) 2-4. transformation Add ligated plasmid (~5 ul) to 50 ulof competent cells (e.g. DH5!) Incubate cells on ice, for 30 min Heat at 42˚C, 30 sec Cool down on ice, for ... WebLigation high Ver.2」は、従来の「Ligation high」をさらに使いやすく改良した、高効 率ライゲーション試薬です。 本製品には、以下のような特長があります。
Toyobo ligation high ver 2
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WebフラグメントA およびフラグメントBそれぞれ200ng、ライゲーション試薬Ligation high Ver.2 (東洋紡)をトータル10μL となるように混合し、16℃で4 時間（または4℃、一晩） ... E-mail : [email protected] 東洋紡株式会社 バイオプロダクト営業部 (東京) … Webgated using Ligation high ver. 2 (Toyobo Co., Ltd.) for 6 hr at 16°C in a reaction volume of 8 μL (4 μL of the digested gDNA was mixed with an equal amount of Ligation high ver. 2). After ethanol precipitation, ligated samples were resolved with 30 μL of TE buffer. PCR was performed in a reac-
WebBio) and ligated using Ligation High Ver. 2 (Toyobo). The ligated DNA was used to transform One Shot PIR1 Chemically Competent E. coli (Thermo Fisher). The transformed E. coli were Web3 [1] Insert insertion ligation 1. Method Five μl of DNA solution was prepared by adding 1,444 bp fragments (5-125 ng, 5-125 fmol) of pUC18/Taq I to dephosphorated pBluescript II/Ban …
Web*2 Adding an equal volume of Ligation high Ver.2 to the DNA solution is recommended in the case of TA cloning. *3 The reaction can be prolonged for up to 2 hr. Standard cohesive … WebToyobo ligation high kit Ligation High Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article …
WebTOYOBO USA INC LIGATION HIGH VER. 2. Manufacturer: TOYOBO USA INC LGK201. This product was recently added by customer request, and is available for your convenience.
WebPrice from $9.99 to $1999.99 ligation high ver 2 ligase - by Bioz Stars , 2024-03 86 / 100 stars Images ligation high ver 2 ( Toyobo ) About News Press Release Team Advisors … pip and council tax supportWeb実験法としては、まず、λDNAの500bpをターゲットにrTaq DNA PolymeraseでPCRを行い、その反応液1μL (約6.4ng)と2μLのTベクター (50ng)を3μlのLigation highに加え、16℃に … pip and csaWebThe steps from terminal repair and 3' end adenylation to adapter ligation can be conducted in the same container. Terminal repair and adenylation at the 3' end can be performed in 15 minutes. Adapter ligation can be done in 15 minutes. Library amplification can be performed in cycles of 10 seconds’ annealing and 15 seconds’ extension. pip and darcyWebLigation was performed using Ligation High Ver. 2 (Toyobo). The sequence of the constructs was confirmed by direct sequencing. Generation ofAk1-OE medaka TheNotI-NotI interval of theAk1-OE plasmid was purified and diluted with sterilized water (final concentration: 20 ng/μL) and then microinjected into the cytoplasm of one-cell stage … pip and crpsWebThe template plasmid was digested using DpnI, and the PCR product was self- ligated using Ligation High with T4 Polynucleotide Kinase (Toyobo). E. coli DH5α were transformed with the ligated product, and the plasmid was pur- ified as described above. pip and dialysisWebLigation high Ver.2 is a highly efficient premixed T4 Ligase reagent. Features Effective ligation of cohesive, blunt and A overhang DNA fragments can be achieved. Will not … The cycling conditions can be set flexibly when various targets having different … Source. E. coli strain that carries the cloned T7 RNA polymerase gene from T7 phage. … dNTPs Mixture (2 mM) dNTPs Mixture (10 mM) Calf Intestine Alkaline Phosphatase; … KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon … dNTPs Mixture (2 mM) dNTPs Mixture (10 mM) Calf Intestine Alkaline Phosphatase; … Typical plasmid yield from an E. coli cell line carrying a high-copy plasmid is approx. 3 … Ligation high Ver.2; TArget Clone/TArget Clone -Plus-10xA-attachment mix; … pip and darcy beaumarisWebOct 17, 2024 · After that, pKLC21 was treated with restriction enzyme BsaI-HF, gel-purified and subsequently ligated with the annealed oligo DNA using Ligation high ver. 2 to obtain pKLC21_bla IMP-1 _104. Likewise, a series of pKLC21 vectors targeting other carbapenem resistance genes (bla NDM-1 , bla KPC-2 , bla OXA-48 , bla VIM-2 stephens college graduate programs