Slowfade gold® antifade reagent invitrogen

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WebbThe sections were then washed with TBS for 15 min, rinsed with distilled water, and mounted on glass slides using SlowFade Gold Antifade Mountant with DAPI (ThermoFisher Scientific, S36937). Sections were imaged on an automated epifluorescent microscope (Zeiss AxioImager Z1) using a 63× Plan-Apochromat 1.4 NA oil objective. WebbManufacturer Thermo Fisher Scientific Model: S36963 Available Worldwide. lowFade® Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. SlowFade® Diamond mountant provides superior antifade protection across the entire visual and IR spectrums.

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Webb20 mars 2024 · Invitrogen™ SlowFade™ Gold Antifade Mountant. Liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. Brand: Invitrogen™ S36940. Code : NEW. Additional Details : Weight : 0.00680kg. View more … WebbThe SlowFadeGold Antifade reagent is a nonhardening mountant that was developed to provide the highest degree of antifade protection for the widest range of dyes and is intended for short-term preservation (3–4 weeks). Using these reagents, samples can be … ct scan for womb cancer

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WebbEvaluation of the morphologic and cellular changes and DNA synthesis post treating Selective Laser Trabeculoplasty of human trabecular meshwork tissue explants. Webb22 mars 2016 · Sections were stained with TO-PRO-3 (1:1000; Invitrogen) for nuclear visualization in HSCs. All sections were mounted with SlowFade Gold antifade reagent (Invitrogen) and observed with a Leica SL confocal microscope under a 100X objective lens (Leica Microsystems) located in the Keck Imaging Facility at the University of … ct scan for therapy guide uses

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Webb3 dec. 2024 · Slides were sealed with SlowFade Gold antifade reagent (Invitrogen) and imaged a Zeiss ApoTome.2 fluorescent microscope (Carl Zeiss) and processed using ZEN software (Carl Zeiss). Antibodies were as follows: CD3ε (550277; BD Biosciences), B220 (RA3-6B2; BioLegend), and LYVE1 (103-PA50; Reliatech); and anti-hamster A546 ... WebbProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image. $101.00 - $323.00 Specifications View More Specs ct scan for vaginal bleedingWebbFixed cells were washed 3 times in blocking solution, 10% bovine serum albumin in PBS, incubated overnight with UCP1 antibody, washed, and incubated with secondary antibody conjugated with FITC. Cells were washed 3 times in PBS and mounted in SlowFade Gold Antifade Reagent with DAPI for nuclear staining (Invitrogen). earthworm anatomy quizlet

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Slowfade gold® antifade reagent invitrogen

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WebbIt contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal. WebbProLong Gold is a hard-setting watery mountant that cures at 1.47 RI in provide photobleach protection and long-term storage of fluorescently labeled fixed cell or tissue samples up till 20 um. ProLong™ Gold Antifade Mountant According to your experiences, what is the best cover slip mounting medium for IF? ResearchGate

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Webb23 feb. 2024 · The slides were washed three times in PBTr, incubated with FITC-, TRITC-coupled secondary antibodies, or with Alexa-fluor 488/562 (donkey anti-rabbit), diluted in PBTr for 60–75 min at RT, washed three times with PBTr, then two times with PBS followed by mounting with SlowFadeÂ Gold antifade reagent with DAPI (Invitrogen, USA), sealed … WebbAbstract. Most spatial transcriptomics technologies are limited by their resolution, with spot sizes larger than that of a single cell. Although joint analysis with single-cell RNA sequencing can alleviate this problem, current methods are limited to assessing discrete cell types, revealing the proportion of cell types inside each spot.

Webb2 jan. 2006 · 1. Introduction. Although C. elegans can mainly recommended for its facile genetics, there has been a burgeoning interest in learn cell biological procedures in these organism. St Webb20 mars 2008 · The Invitrogen Corporation markets the SlowFade Gold® Antifade Reagent which is an improved version of their original SlowFade® Reagent. SlowFade Gold® was designed to increase the photostability of many different fluorophores used when …

WebbAntifade reagent, such as SlowFade® Gold (S36936) or ProLong® Gold (P36930) antifade reagents OR Flow Cytometry PBS Ethanol Tris staining buffer (see step 4.1) OR Chromosome FISH dH 2 O PBS RNase A Antifade reagent, optional Making a Stock Solution from Solid PI To make a stock solution from the solid form, dissolve PI (MW = … Webbfor SlowFade® Gold and ProLong® Gold reagents are available on our website at probes.invitrogen.com. Materials Kit Contents • SlowFade® antifade reagent (Component A), 10 mL in 50% glycerol (v/v) ready-to-use and sufficient for at least 200 coverslip-size …

Webb7 dec. 2006 · Reagent Setup 0.3% (vol/vol) PBT Add 1.5 ml Triton-X 100 to 498.5 ml PB. Store this nonhazardous buffer at room temperature (20–25 °C). Different staining procedures or antibodies may require...

Webb9 mars 2024 · After washing with PBS, cells were counterstained with 500 nM propidium iodide solution. Slides were mounted using the SlowFade Gold antifade reagent (Invitrogen, CA, USA). Confocal fluorescence imaging was performed using the Carl Zeiss LSM 510 META confocal microscope (Carl Zeiss Inc., Jena, Germany). earthworm anatomy and functionsWebbFluoromount has a RI of 1.40 while prolong antifade has a refactive index of 1.52. You should match the RI of the mounting solution with the immersion oil. The RI of the oil and cover slip are... ct scan for stomach cancerWebbSlowFade® Gold and SlowFade® Diamond Antifade Mountants are intended for short-term use (3–4 weeks); fluorescence signal from samples mounted using this reagent may degrade over time. Technical specifications for SlowFade® Gold and SlowFade® Diamond Antifade Mountants • pH 7.4 at 20°C • Refractive index is 1.42 at 20°C Experimental ... ct scan for thoraxWebbLate-onset Alzheimer's disease (AD), the most prevalent neurodegenerative disorder in the aging population, is increasing due to higher life expectancy, and an adequate treatment remains elusive. ct scan for shoulder painWebbProlong および Slowfade 褪色防止用封入剤シリーズ Invitrogen™の褪色防止用封入剤は、どれもプレミックスタイプでそのまま使用可能です。サンプルに1～2滴加えてマウントするだけです。製品ごとに下記の特長を持ちます。ご要望に合わせて最適な製品をご使用ください。 Invitrogenから初めてのライブセルイメージング用褪色防止剤 ct scan for the kidneysWebbInvitrogen™ ProLong™ Gold Antifade Mountant with DNA Stain DAPI Catalog number: P36931 Related applications: Cellular Imaging Technical Support Customer Service ProLong Gold Antifade Mountant with DNA … ct scan for sternal fractureWebbThe cochleae were immediately immersed in ice cold 500 μL Trizol Reagent (Invitrogen/Ambion) ... tissue underwent final trimming to ensure the specimens were flat and then coverslipped with SlowFade Gold with DAPI (Invitrogen, ... Wholemount cochleae were mounted on microscope slides with antifade FluoroGold with DAPI (Pierce/Fisher, … ct scan for stomach pain