Pmon530

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August undefined, 2024

Webrived from the binary vector pMON530 [15]. In designing the T-DNA of the binary vector the primary aim was to provide an organisational structure with a IacZ' region immediately 3' of the right T-DNA border and 'overdrive' enhancer … WebAug 16, 2024 · To investigate the subcellular localization of OpWRKY3, its full length of OpWRKY3 was amplified and inserted into vector pMON530-GFP located at the restriction site of Bgl II and Kpn I to generate pMON530-OpWRKY3-GFP. The constructed expression vector was transferred into Agrobacterium strain ASE and injected into 60-day old …

Frontiers Transcription Factor SmWRKY1 Positively Promotes …

WebpMON530-GFP, E. coli MM294 carrying a helper plasmid pRK2013, and A. tumefaciens GV3111 containing the Ti-plasmid pTiB6S3SE. Recombinant A. tumefaciens GV3111 WebAug 16, 2024 · To investigate the subcellular localization of OpWRKY3, its full length of OpWRKY3 was amplified and inserted into vector pMON530-GFP located at the restriction site of Bgl II and Kpn I to generate pMON530-OpWRKY3-GFP. The constructed expression vector was transferred into Agrobacterium strain ASE and injected into 60-day old … asbenz kuala terengganu

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WebMar 1, 2024 · This was then ligated into the pMON530-GFP vector, in frame with green fluorescent protein (GFP) under the control of the CaMV 35S promoter. The resultant pMON530-TCM1-GFP plasmids were introduced into tobacco (Nicotiana tabacum L.) leaves and cocultured at 25°C for 2 d. In the meantime, pMON530-GFP empty vector was used … WebVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited at Addgene are available for purchase through this website. WebFeb 15, 2024 · To examine the subcellular localization of SmERF1L1, the full-length ORF of SmERF1L1 was amplified and cloned into pMON530 vector between the BglII and KpnI sites to generate the expression vector pMON530-SmERF1L1-GFP under the control of a constitutive CaMV35S promoter. asbenz kuantan

The rice TCD11 encoding plastid ribosomal protein S6 is essential …

Molecular cloning and characterization of two 1-deoxy-

WebNov 18, 2014 · As a control, vector DNA containing the CaMV 35S promoter alone (pMON530) was introduced into Agrobacterium. Agrobacterium infusion assays and RNA isolation. Vacuum infiltration of Arabidopsis thaliana plants with Agrobacterium cultures was performed essentially as described . WebDec 1, 2016 · The authors are grateful to Prof. Zhongnan Yang (Shanghai Normal University) and Yaoguang Liu (State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University) for kindly providing pMON530-GFP vector, and the pYLgRNA-OsU6 and pYLCRISPR/Cas9-MH) vectors, … asberaWebOct 2, 1992 · The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function. All Science Journal Classification (ASJC) codes asber aerb-12

"WebJan 14, 2010 · The resulting plasmid pMON530-CLS was first introduced into Agrobacterium tumefaciens by electroporation and then transformed into the Arabidopsis las mutant by floral dip method (Clough and Bent 1998). Transgenic plants were selected on MS medium containing 20 mg/L Kanamycin. DNA Isolation and Gel Blot Analysis " - Pmon530

Pmon530

Transcription Factor OpWRKY3 Is Involved in the Development …

WebNational Center for Biotechnology Information WebMar 21, 2016 · The resultant pMON530-TCM5-GFP plasmids were introduced into tobacco (Nicotiana tabacum) leaves and co-cultured at 25 °C for 2 days. At the same time, the pMON530-GFP empty carrier was used as a control. Then the GFP fluorescences in tobacco cells were observed using a Zeiss confocal laser scanning microscope ...

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Webin pDR195 or with EcoRI for cloning in pMON530 (Monsanto). Yeast Strains and Growth Conditions. The strains used in this study were INVSc1 (Invitrogen), DEY1453 (17), and smf1 (MATahis3 ade2 leu2 trp1 ura3smf1::URA3ura3::TRP1). This smf1 strain was derived from the smf1 from Supek et al. (22) by transformation with WebApr 9, 2015 · The pMON530:CaMV35S:ASL3-GFP plasmid was introduced into tobacco cells using Agrobacterium-mediated infection method. Meanwhile, empty GFP vector was used as a control. As a result, the green fluorescent signals of ASL3-GFP fusion protein perfectly overlapped with chloroplast autofluorescence in transformed tobacco mesophyll …

WebThe constructed expression vector pMON530-SmERF1L1-GFP. The AP2/ERF transcription factor SmERF1L1 regulates the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza WebMay 1, 2024 · The resultant pMON530:CaMV35S:TCD33-GFP plasmid was confirmed by sequencing and introduced into the Agrobacterium strain EHA105. The localization of TCD33 was investigated by transient expression assays of the GFP fusion protein in tobacco ( Nicotiana tabacum ) cells by laser scanning confocal microscopy (LSCM) as described …

WebAug 25, 2016 · a pMON530-GFP, used as the blank vector control; b pMON530-SmDXS1-GFP; c pMON530-SmDXS2-GFP. Full size image. PCR and qRT-PCR analysis of transgenic hairy roots. The observed differential expression of the two SmDXS genes suggested that they perform distinct regulatory roles in the first step of the MEP pathway. WebApr 25, 2000 · 35 S-AtNramp3 (filled bars): homozygous, single insertion line transformed with pMON530 containing AtNramp3 cDNA driven by the 35 S CaMV promoter. To determine whether AtNramp3 affects Fe accumulation, we measured the iron content of control and AtNramp3 overexpressing seedlings grown in minimal medium …

WebMay 15, 2006 · This DNA fragment was cloned into a T-vector (TaKaRa, Japan), verified by sequencing, and inserted into the plant transformation vector pMON530 (Monsanto, USA), downstream to the 35S promoter.

WebMay 30, 1993 · These DNA fragments have been cloned into the binary vector, pMON530, such that either the nopaline synthase (Nos) promoter or cauliflower mosaic virus (CaMV) 35S RNA promoter is used to drive synthesis of the corresponding sense and AS RNAs. Transgenic Nicotiana tabacum cv. Xanthi nn plants containing these constructs were … as beogradWebDot blot assay of transgenic (Tr1,Tr2,Tr3) tomato plants and plasmid pMON530 (P). Membrane probed with Alk Phos labelled CKI-1 gene PCR fragment Source publication Differential response of tomato... asber aerb-24 asbenz sungai petaniWebfragment from the plasmid pMON530 in Escherichia coli strain NM294 was used as the probe for transformed tissue DNA. Plas-mid DNA was isolated from E. coli using an alkaline lysis method (Liszewski et al., 1989; Sambrook et al., 1989). PCR amplification of the nos fragment for probe preparation or for plant DNA analysis was carried out ac- asbepalingWebDownload scientific diagram Schematic representation of the T-DNA region of the binary vectors pMON337-TGMV-GFP and pMON530-GFP. asbera loginWeb本发明涉及基因工程技术领域，具体而言，涉及草莓hy5基因在调控草莓果实成熟周期中的应用、过表达载体及方法。该方法为构建含有草莓hy5基因的过表达载体，将所述过表达载体导入宿主菌，采用所述宿主菌对草莓果实进行瞬时遗传转化。hy5基因的过量表达可以使草莓果实延迟成熟，实现草莓经济 ... asbepaling ecgWeb7.根据权利要求6所述一种采用草莓hy5基因调控草莓果实成熟周期的方法，其特征在于，所述过表达载体为pmon530-hy5。8.根据权利要求6所述一种采用草莓hy5基因调控草莓果实成熟周期的方法，其特征在于，所述宿主菌为农杆菌gv3101感受态。 asberg surname