Phospho eif2 alpha antibody
WebMay 13, 2024 · For eIF2 phosphorylation, purified PKR and eIF2 (0.08 µg PKR per 10 µg of eIF2) were mixed at room temperature in eIF2 storage buffer supplemented with 0.5 mM ATP, 10 mM MgCl 2 and 5 mM NaF, and ... WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) suitable for the …
Phospho eif2 alpha antibody
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http://immunoway.com/Home/22/YP0093 WebAnti-EIF2 α Antibody detects endogenous levels of total EIF2 α protein. Immunogen The antiserum was produced against synthesized peptide derived from human eIF2 alpha. Immunogen Range: 21-70 Features and Benefits Evaluate …
WebIHC-P analysis of human breast carcinoma tissue using GTX50300 eIF2 alpha (phospho Ser51) antibody. GTX50300 WB Image WB analysis of K562 cells untreated or treated with IFN-α (100ng/ml, 20min) lysates using GTX50300 eIF2 alpha (phospho Ser51) antibody (Lane 3 and 4) and eIF2α antibody (Lane 1 and 2). ... WebProduct Name eIF2 alpha (Phospho-Ser51) Rabbit mAb; Host Species Rabbit; Clonality Monoclonal; Isotype Rabbit IgG; Purification Affinity-chromatography; Applications WB …
WebBinding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). WebPhosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important …
WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ...
WebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ... how to run a page likes campaign on facebookWebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. Validations None provided Applications Western Blotting (WB) Reactivity Chlorocebus sabaeus (Green monkey) how to run a packageWebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm … northern ontario travel grant printableWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … how to run a p32 on sageWebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … how to run a one room schoolhouseWebPhospho-EIF2S1 (Ser51) antibody for WB, ELISA and reacts with Human, mouse, rat. ... eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51 ... EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis ... how to run apache server on windowsWebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated … how to run anything as admin