Phic31 attb

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August undefined, 2024

WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly …

PhiC31/pUAST-mediated site-specific transgenesis in

WebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. WebMar 21, 2012 · Depending on the relative orientation of the attP and attB sites, the reaction can result in excision, inversion or integration of sequences between the recognition sites, and is not reversible unless an additional protein, an excisionase, is present. carbonated glycerin

PhiC31 Integrase Expression Plasmid System Biosciences

WebphiC31 attB site Species phage phiC31 Insert Size (bp) 285 Cloning Information Cloning method Gateway Cloning 5′ cloning site SalI (not destroyed) 3′ cloning site HindIII (not destroyed) 5′ sequencing primer M13 -21 3′ sequencing primer M13 reverse (Common Sequencing Primers) Terms and Licenses Academic/Nonprofit Terms UBMTA Industry … WebMay 7, 2013 · We showed in our previous paper that phiC31 integrase works very efficiently in silkworm embryos using an extra chromosomal cassette exchange reaction system; recombination between the attP and attB sites of the plasmid occurred with a high frequency in the silkworm embryos when phiC31-integrase mRNA was supplied by … WebpUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494) Plasmid #160577. Purpose. 35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB) Depositor. Diego Orzaez. Article. Bernabe-Orts et al Nucleic Acids Res. 2024 Apr 6;48(6):3379. carbonated gatorade

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WebSearch $34 million in missing exemptions going back four years. Change your name and mailing address. Pay Online for Free. Use your bank account to pay your property taxes … WebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat … broadway variety south portland maineWebHow it works: Because the two sites recognized by the PhiC31 integrase differ and the recombination process in essence mixes the two sites into two different sites (attR and attL), &C9B8;C31 based integration is unidirectional. attB + attP + … broadway va to front royal va

"WebJul 21, 2006 · The stability of the attB × attP locus, well-documented with the phiC31 integrase system in bacteria and yeast 16,17, was evidenced here by the lack of rearrangement or reversion to the wild-type ... " - Phic31 attb

Phic31 attb

Sequences of PhiC31 and BxB1 attachment sites For …

WebOct 28, 2024 · 7, when a cell containing the variant phiC31 integrase that recognizes the pseudo-attP site, a recombination between the pseudo-phiC31 attP site and the phiC31 attB site occurs and the orientation of the CMV promoter in the selection construct is reversed. The reversed CMV promoter then drives the expression of the GFP reporter gene. WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between …

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WebThe key to the PhiC31 integrase system is non-random integration. Isolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) …

WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB and attP 2, 3. PhiC31 integrase does... WebFor installation in small housings, control cabinets and operator panels. Simple and fast wiring by means of screw terminals. Space-saving design. For integration of mechanical …

WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ... WebDuring pUAST/PhiC31 integrase-mediated integration, the unique attP site in the ZH-attP-51D fly line genome recombines with the pUAST attB site in the pUAST-PrP vector forming attR and attL...

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WebPlasmid w+attB from Dr. Jeff Sekelsky's lab contains the insert phiC31 attB, hsp70::w. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. … broadway vascularWebEnter the email address you signed up with and we'll email you a reset link. broadway vascular tinton falls njWebThe forward and reverse primers will be designed to incorporate attB1 and attB2 sites respectively, to allow clonase-mediated integration of the PCR product into a Gateway entry vector. The primers will be designed to both have a … broadway vascular san antonio txWebSep 8, 2013 · Phage PhiC31 integrase integrates attB-containing plasmid into pseudo attP site in eukaryotic genomes in a unidirectional site-specific manner and maintains robust transgene expression. Few studies, however, explore its potential in livestock. This study aims to discover the molecular basis of PhiC31 integrase-mediated site-specific … broadway va weather radarWebMar 4, 2016 · In the presence of phiC31 integrase and a plasmid bearing a phiC31 attB site, integration of the plasmid can occur at a pseudo att site, usually in single copy (lower). Integration at pseudo att sites is less precise than at genuine attP sites, and small deletions in the vicinity of the integration site often occur Full size image broadway vascular san antonioWebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. broadway va to richmond vaWebMar 23, 2024 · 该研究一个重要的创新点是通过改造酵母双杂交系统的载体质粒(Yang et al., 2024), 将诱饵蛋白所在载体(pGBKT7)插入ATTB位点, 将捕获蛋白所在载体(pGADT7)插入ATTP位点, 分别在2个载体上插入来自噬箘体PhiC31的表达盒。利用PhiC31介导的ATTB位点和ATTP位点发生重组, 使互作的2 ... broadway va to luray va