P-chk1 antibody
SpletAnti-CHEK1 (Ab-317) antibody produced in rabbit is polyclonal, unconjugated and is suitable for immunohistochemistry, western blot US EN Applications Products Services … SpletChk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345. The Life Science industry has been in the grips of a …
P-chk1 antibody
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SpletThe slurry contains the bound CHK1 antibody and CHK1 protein, and any loss of this sample will affect the final results. 2. Add 20 μL of kinase buffer containing 2 μg of CDC25C protein and 5 μCi 32 P γ-ATP. In order to have an equal volume of substrate and isotope activity in each sample, ... SpletCheckpoint kinase 1, commonly referred to as Chk1, is a serine/threonine-specific protein kinase that, in humans, is encoded by the CHEK1 gene. Chk1 coordinates the DNA …
Splet30. dec. 2008 · Cells treated with 0.5 mM HU for 3 h were lysed and probed with phospho-Chk1-specific antibodies, or with an antibody against total Chk1 protein, as indicated. α … SpletAnti-phospho-Chk1 (pSer286) antibody produced in rabbit affinity isolated antibody; Synonyms: CHEK1,CHK1,CHK1 checkpoint homolog; find Sigma-Aldrich-SAB4301484 …
SpletAntibody: Tissue Type: 8-OHdG. AACT. AAT. ACTH. AE-1. AE-3 . AE-1/AE-3. Actin, Muscle. Actin, Alpha-Smooth Muscle. AFP. Albumin. Androgen Receptor. Aurora A/STK-15 ... Splet01. jul. 2024 · 1. Introduction. The anti-PP1Pk, previously known as anti-Tja, is a natural occurring antibody against P, P1 and Pk red cell antigens. Individuals in which these …
SpletWestern blot - Phospho-Chk1-S345 Rabbit pAb (AP0578) Western blot analysis of extracts of C6 cells, using Phospho-Chk1-S345 pAb (AP0578) at 1:1000 dilution or Chk1 antibody (A7653).C6 cells were treated by ATP (5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
SpletChk1 (phospho Ser345) stained by Phospho-CHK1 (Ser345) Polyclonal Antibody (Product # PA5-34625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. Phospho-CHK1 (Ser345) Polyclonal Antibody detects Chk1 (phospho Ser345) protein at nucleus on mouse colon by immunohistochemical analysis. remove background imagemSplet21. feb. 2024 · e Cells treated with the indicated inhibitors were fixed at 2 h after UV irradiation and immunostained with an anti–p-CHK1 antibody. In each experiment, >100 cells were randomly chosen to ... lago mar westin homesSplet19. dec. 2011 · This band was not recognized by the G4 monoclonal antibody from Santa Cruz Biotechnology that is commonly used for immunoblot ... JM Raleigh, HM Verkade, P … lago open road snowboardSpletCHK1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). CHK1 plays an essential role in the mammalian DNA damage checkpoint, embryonic … lago shelves italySplet30. jun. 2024 · (C) SAβG staining and antibody staining for γH2AX, H3K9me3, p-H3, and cell apoptosis detected by a TUNEL assay. Quantification of antibody staining is shown in (D). Protein abundances of p21 (E), p-Chk1 and total Chk1 (F), p-Chk2 and total Chk2 (G), γH2AX and total H2AX (H), p53 (I), and ZEB1 and ZEB2 (J) in lago plasir dark chocolate wafers 25x38gmSpletBethyl Laboratories Anti-Chk1 Polyclonal, Catalog # IHC-00004. Tested in Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse … remove background image from bing homepageSpletThe AlphaLISA® SureFire® Ultra™ p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 … lago mar reservations