WebHiTrap Desalting 5 ml column, a HiPrep™ 26/10 Desalting column, or dialyzed against PBS to remove glutathione, which can interfere with the protein measurement. The Bradford method can be used in the presence of glutathione. • The reuse of GSTrap 4B depends on the nature of the sample and should only be performed with identical samples to Web8 The purified fractions can be buffer exchanged using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns if necessary (Table 4). Note: The reuse of HiTrap Protein G HP depends on the nature of the sample and should only be performed with identical monoclonals to prevent cross-contamination.
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WebHiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns if necessary (Table 5). Note: The reuse of HiTrap rProtein A FF depends on the nature of the sample and should only be performed with identical monoclonals to prevent cross-contamination. 10 71500273 AL Table 5. Web23 feb 2024 · Alternatively, a two-step chromatography purification was performed by ion exchange chromatography on a HiTrap™ Q FF (50 mM BisTris containing 30 mM NaCl (buffer A) and 1 M NaCl (buffer B), pH 6.5, gradient 12–100 % buffer B) followed by size-exclusion chromatography in Tris-buffered saline, pH 7.9 on a HiLoad™ 16/600 … city of lincoln ri
HiPrep 26/10 Desalting - Cytiva
WebHiTrap™ Protein G HP is a prepacked ready to use, column for purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA™. WebHiTrap Desalting can be used with aqueous solutions in the pH range 2–13. It is stable to all commonly used buffers, solutions of urea (8 M), guanidine hydrochloride (6 M), and all … Webbuffer or by buffer exchange using HiTrap Desalting, HiPrep™ 26/10 Desalting or PD-10 Desalting columns, see Table 4. The sample should be fully solubilized. We recommend … city of lincolnton jobs