Webvarious fluorochromes run on a BD™ LSR II flow cytometer. This chart is meant as a guideline of relative stain indices of various fluorochromes. Observed relative stain … Web520 nm) Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical …
BD BiosciencesFluorochrome Reference Chart
WebEach fluorochrome has distinct properties and is characterized by specific excitation and emission wavelengths. First, the fluorochrome must be excited by the lasers available … WebFigure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 690 dye. Click image to enlarge Figure 2. Spectral signature of NovaFluor Yellow 690 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 690 dye were used for analysis. great falls mt animal control phone number
A guide to fluorochromes Abcam
WebOur new fluorochrome chart includes. Aligned emission and excitation fluorescence spectra for 30 of the most commonly-used flow cytometry fluorochromes including tandem dyes. Easy visualization of some of the most popular lasers and filters across the fluorescence spectra. The fluorescence channel and relative brightness for each of the ... WebThe following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. They are intended to provide a … Webnm, with an emission peak wavelength of 509 nm. The wild type like variants have their primary excitation peak centered on 395 nm, with an emission peak at 509 nm while the blue emitting mutants generally have an excitation peak at around 380 nm and an emission peak near 460 nm (Figure 1). Table 1. Excitation and emission data of GFP variants GFP great falls mt asl interpreting services