Alexa phalloidin 488
WebNov 9, 2024 · Typically, phalloidin is used conjugated to a fluorescent dye, such as FITC, Rhodamine, TRITC or similar dyes, such as Alexa Fluor® 488 or iFluor 488. Phalloidin … WebAnti-rat IgG (H+L) antibody was conjugated to Alexa Fluor ® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This product has been optimized for use as a secondary antibody in immunofluorescent applications.
Alexa phalloidin 488
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Web7 rows · Alexa Fluor® 568 Alexa Fluor® 647 ... CoraLite® Plus 488-Phalloidin (green) Ex/Em: 490/515nm. Storage. Store at -20°C. Avoid exposure to light. Stable for 2 years … WebAlexa Fluor 488-phalloidin. Nanometer scale channels were created within the cytoskeleton by translating the cell at 5 ...
WebNormal Mouse IgG, Alexa Fluor® 488 conjugate. Compare. Product No. Description. SDS. Pricing. 16-240. Normal Mouse IgG Polyclonal Antibody control validated for use in … WebActinGreen 488 reagent is formulated as a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) and comes in a convenient dropper bottle. Just tip and …
WebAlexa Fluor 488 phalloidin Solutions needed 10% TritonX-100 (prepare the night before): 45 mL phosphate-buffered saline (PBS) + 5 mL 10% TritonX-100 Fixation solution (see below) DAPI and phalloidin in PBS (1:1000 dilution) Fixation solution 3.25 mL PBS 500 µL TritonX-100 (10%) 6.35 mL PFA (32% paraformaldehyde) PBT (0.1%) 50 mL PBS WebAlexa Fluor® 488 Phalloidin can be diluted 1:20 (5 μl per 100 l assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once …
WebSep 17, 2024 · If performing additional antibody staining, DAPI and Alexa Fluor 488 phalloidin can be detected using the FITC, and an additional antibody of interest can be detected using the red channel. Note: Maintain consistent laser strength and detection settings across all samples being compared.
WebHuman peripheral mononuclear cell and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA) and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. disease of the public mindWebAlexa Fluor Hg‐Link dye is a new generation of fluorescent probes that exhibit some advantages over other fluorescent dyes, such as being highly fluorescent over a broad pH range (pH 4 to 10), good water solubility, and higher photostability, allowing more time for … disease of the sensesWebApr 8, 2024 · F-actin and nuclei were labeled with Alexa Fluor 488 phalloidin (green) and 4’,6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 50 μm. f, g Fluorescence imaging of EGFP expression (f) and percentages of EGFP-positive cells (g) in B16-F10 cells and the other 7 types of cells after NP Tyr-C9AP transfection. Scale bar, 50 μm. disease of the spinal cord is calledWebJan 17, 2024 · Phalloidin staining and confocal microscopy Cells were plated on confocal dishes coated with poly-L-lysine. After incubation at 37 °C for 24 h, cells were fixed with 4% paraformaldehyde for 30 min, and were stained with 5 μg/mL Alexa Fluor 488-phalloidin (Invitrogen) to reveal filamentous actin (F-actin) in PBS for 40 min at 37 °C. disease of the spinal cord icd 10WebAlexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Invitrogen™ Alexa … disease of the testes medical termWebFinally, the cells were stained with Alexa Phalloidin 555, to bind the actin filaments and highlight the cell morphology. ... (488 nm). The zeta potential of the hybrid Rh-CQDs NPs was estimated as −28 ± 1 mV, revealing the presence of carboxyl groups on the surface of the hybrid NPs, even after the conjugation process. Moreover, the ... disease of the testesWebMar 30, 2024 · For actin staining, Alexa Fluor 488 phalloidin (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented with 0.1 μg/mL DAPI (Sigma-Aldrich). Cells were observed on confocal microscope (Leica TCS SP8) with HC PL APO 63×/1.40 oil … disease of the prostate